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KMID : 0613820070170020223
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Journal of Life Science
2007 Volume.17 No. 2 p.223 ~ p.229
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Temporal and Spatial Role of Pupal Stage Specific Cuticle Protein in Artogeia rapae
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Shin Myung-Ja
Park Jeong-Nam
Seo Eul-Won
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Abstract
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Present study aims to investigate the topical distribution of pupal stage specific cuticle protein and its temporal and spatial role during the wing formation of Artogeia rapae. ArCP27 (27 kd cuticle protein) was identified as pupal stage specific cuticle protein in cuticle tissues and has not shown any qualitative differences by local portions of body. ArCP27 maintained constant concentration just after pupal ecdysis to 5-day old pupal stage but thereafter decreased. In fat body, ArCP27 was found in both thoracic and abdominal fat body from the last larval to pupal stage. In wing cuticle, ArCP27 began to find from 5-day old pupal stage. Immunologically ArCP27 in thoracic and abdominal cuticle has the response against the ArCP27 at 5-day old pupa but since then has no response. But the antibody against ArCP27 has reacted to 5- and 7-day old pupal and adult wing protein. 3H-leucine was not incorporated into ArCP27 in 5- and 7-day old thoracic and abdominal cuticle but was incorporated into ArCP27 in 7-day old wing cuticle and adult wing, suggesting that ArCP27 partly participates the wing cuticle formation by the process of digestion and reabsorption of old cuticle.
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KEYWORD
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cuticle, wing, ArCP27, stage specific cuticle protein
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